Studies of type 2 dm models should provide further understanding of the underlying mechanisms of diabetic atherosclerosis. Diabetes was induced in the rabbits with alloxan monohydrate of 150 mgdl body weight except normal and control. Introduction diabetes mellitus diabetes is a group of metabolic diseases in which a person has high blood sugar, either because the body does not produce enough insulin, or because cells do not respond to the insulin that is. Alloxaninduced diabetes causes morphological and ultrastructural. Experimental diabetes induced by alloxan and streptozotocin.
Fresh liver weight and its relationship with body weight were obtained, and liver tissue was. Alloxan has been administered in single or multiple. Pdf alloxaninduced diabetes, a common model for evaluating the. It was the first agent used in the category of chemically induced diabetes to create a model of insulin dependent diabetes mellitus. A journal of synthetic chemistry and natural product chemistry. The resulting insulinopenia causes a state of experimental diabetes mellitus called alloxan diabetes 4, 8, 9. By continuing to use our website, you are agreeing to our use of cookies.
The aim of these experiments was first to follow the growth of ascitic or solid ehrlich tumor in relation to the number of transplanted tumor cells and to the dose of alloxan injected to induce diabetes before the tumor. Alloxan is reduced to dialuric acid and reoxidized to alloxan producing alloxan radicals and reactive. Managing diabetes devoid of any adverse effect remains an issue to health system. The effect of aepe leaf extract on blood glucose, insulin, hemoglobin and. Extract ethanol of poguntano in alloxan induced diabetic rats. Altered glucose metabolism and hypoxic response in alloxan. I1 1department of medical laboratory science, school of basic medical sciences, university of benin, nigeria 2health services department, university of benin, nigeria 1. In this study 36 male wistar rats, body weight of 150200gr were divided into 6 groups.
Serum samples were analyzed by radioimmunoassay for progesterone p and 17. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Conflicts of interest e author declares that he has no conicts of interest. Rabbits having blood glucose level above 250 mgdl in fasting condition were chosen for the study as diabetes rabbits.
Research article alloxan induced diabetes mellitus in. This research was designed to investigate antihyperglycaemic impact of aqueous extract of moringa oleifera leaf on alloxaninduced diabetic male wistar rats. Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals. Diabetes mellitus is a metabolic disorder characterized. Raw camel milk properties on alloxaninduced diabetic wistar rats. Thirty nondiabetic control rats nc and 30 untreated diabetic ud rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks. This study evaluated the longterm effects of alloxaninduced diabetes in rat liver.
Alloxaninduced diabetes, a common model for evaluating the glycemiccontrol. Dec 18, 2007 alloxan has two distinct pathological effects. The reduction product of alloxan, dialuric acid 1, has also been shown to be diabetogenic in animals 10, 11, and to cause ultrastructural changes identical to those observed in response to alloxan 6. Alloxan induced rats,anti diabetes, diabetes, ipomoea batatas, phytochemical analysis. Although progress has been made to show the role of raw flaxseed and flaxseed extracts in health promotion, identification of mechanisms of action and molecules underpinning beneficial effects largely remain unknown. W m vogel and c s apstein houseman medical research center, department of pharmacology, boston university school of medicine, ma 021182394. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. The methanolic whole plant extract at high dose 500 mgkg exhibited significant antihyperglycemic activity than whole plant extract at low dose 300 mgkg in diabetic rats. The mechanisms of alloxan and streptozotocininduced.
Albino rats each weighing 180230g were divided into 3 equal groups n10 as following. Alloxan has been administered in single or multiple doses, through different routes intraperitoneal, intravenous and subcutaneous. Fresh liver weight and its relationship with body weight were obtained, and liver. Alloxan monohydrate is used to induce diabetes mellitus in albino rats in the dose of 120mgkg i. Pdf experimental diabetes induced by alloxan and streptozotocin. The mechanism of alloxan and streptozotocin action in b cells. Our results showed that daily supplementation of vco 20% of food for 16 weeks significantly p alloxan induced diabetes. Diabetes is a metabolic disease linked to weakened sugar metabolism. Diabetes mellitus is a group of metabolic disease with high blood glucose. The protective role of virgin coconut oil on the alloxan. Antidiabetic effect of ipomoea batatas in normal and alloxan. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals 78, 79. The present study evaluated the efficacy of an aqueous flaxseed extract afe to correct alloxaninduced diabetes in mice. Diabetes was induced in wistar rats n 25 by intravenous administration of alloxan 42 mgkg and were analyzed at 1, 3, 6, 9 and 12 months after diabetes induction.
Effects of alloxaninduced diabetes on corpus luteum function. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t. Histologically, the pancreatic acini were diffusely atrophied, and the islets showed marked. Diabetes was induced by intra peritoneal injection of 120 mgkg alloxan. Second, by transplanting tumor cells from primary diabetic recipients into secondary. Seven male f344 rats with diabetes induced by a single i. They were found to be expressly effective in controlling diabetes and its. Garlic in alloxaninduced diabetic rabbits, the american journal of clinical nutrition, volume 28, issue 7, 1 july 1975, pages 684685, htt we use cookies to enhance your experience on our website. Therefore, there is an increase in the search for better antidiabetic medicine. Growthandtreatment of ehrlichtumorin micewith alloxaninduced. Alloxan is a urea derivative which causes selective necrosis of the. Alloxan induced diabetic rats emili augustina mmaduaburochukwu1, osadolor h. In 1943, alloxan became of interest in diabetes research when dunn and mcletchie reported that it could induce diabetes in animals 4 as a result of the specific necrosis of the pancreatic beta cells 57. Alloxaninduced diabetes, a common model for evaluating the.
Alloxan has been administered in single or multiple doses, through different routes intraperitoneal. Journal of diabetes and endocrinology academic journals home. Alloxan induced diabetic rabbits as animal model for diabetes. Giant mitochondria in pancreatic acinar cells of alloxan. Comment on anion gap toxicity in alloxan induced type 2. In research it is used for induction of type 1 diabetes. The effects of alloxaninduced diabetes mellitus on rat ovarian structure and function were examined throughout pseudopregnancy psp. Alloxan induced diabetes is a model of type 1 dm, but type 2 dm is a more common risk factor for cvd. Three days later diabetes was confirmed using one touch glucometer. Journal of diabetes and endocrinology academic journals. Studies on the administration of vernonia amygdalina del. Garlic in alloxaninduced diabetic rabbits the american. In diabetes mellitus the body cannot secrete enough insulin or it cannot use its. The mechanisms of alloxan and streptozotocininduced diabetes.
Diabetes was induced in rats by intravenous injection of alloxan monohydrate dissolved in normal saline into the dorsal tail vein at. Journal of diabetes and endocrinology antihyperglycaemic. Anti diabetic effect of cherries in alloxan induced. Alloxan is a cyclicurea derivative, which is a highly effective, diabetogenic agent used widely as a tool to induce experimental diabetes. Sugarlowering drugs for type 2 diabetes mellitus and. Alloxaninduced diabetes delays repair in a rat model of. Avocado pear persea americana seed, alloxan, diabetes, serum glucose. Improvement of diabetes symptoms and complications by an. Grapefruit juice in alloxaninduced diabetes in rabbits.
Brazilian journal of medical and biological research 2008 41. This study evaluated the longterm effects of alloxan induced diabetes in rat liver. Grapefruit juice in alloxan induced diabetes in rabbits badreldin h ali, suliman i sharif diabetes care aug 1995, 18 8 1199. This study aimed to create diabetic animal models and to provide an exact and detailed account of alloxan induced diabetes in rabbits. Effects of alloxaninduced diabetes on corpus luteum. Hearts from rabbits with 816 weeks of alloxan diabetes were compared with hearts from normal rabbits to determine whether diabetic myocardium is more. Alloxan monohydrate is a common drug used for developing experimental diabetes in animals, as streptozotocin, though less toxic than alloxan is considerably costlier. Antioxidant enzymes and lipid peroxidation in alloxaninduced. Streptozotocin is more specific to beta cells rather than alloxan. Article pdf available in journal of pharmacological and.
Induction of diabetes diabetes mellitus was induced by intraperitoneal injection of 150 mgkg body weight of alloxan monohydrate, suspended in distilled water. Effects of aqueous avocado pear persea americana seed. The aim of this study was to investigate the potential protective effect of virgin coconut oil vco on oxidative stress parameters in the liver, kidneys and heart of alloxaninduced 150 mg kg. Atpase activities were measured in sciatic nerves from rats with alloxaninduced diabetes alxd of various duration 2 wk, 5 wk, 9 wk, and 6 mo. Diabetes was induced in one of the groups, by intraperitonial administration of alloxan at a dosage of 150mgkg body weight and the control rabbits were administered normal saline 0. Group i constituted the control group and group ii constituted the alloxaninduced diabetic group. What are the advantages of streptozotocin over alloxan in. Effects of alloxaninduced diabetes on ischemiareperfusion. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals.
Hypoglycemic effects in alloxaninduced diabetic rats of the. Apstein hearts from rabbits with 816 weeks of alloxan diabetes were compared with hearts from normal rabbits to determine whether diabetic myocardium is more sensitive to ischemic injury. Diabetes follows the alloxaninduced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet. The animals were monitored for 14days after confirmed diabetes in the test group. Streptozotocin is an antimicrobial agent and has also. Alloxaninduced rats,antidiabetes, diabetes, ipomoea batatas, phytochemical analysis. Alloxaninduced diabetes, a common model for evaluating the glycemiccontrol potential of therapeutic compounds and plants extracts in experimental studies medicina, vol. The hemimandibles were removed and submitted to radiographical and histopathological procedures. This research was designed to investigate antihyperglycaemic impact of aqueous extract of moringa oleifera leaf on alloxan induced diabetic male wistar rats. Atpase activities were measured in sciatic nerves from rats with alloxan induced diabetes alxd of various duration 2 wk, 5 wk, 9 wk, and 6 mo.
All animals were obtained from the animal house of medical research center and bilharzial unit, faculty of medicine, ain shams university. It has been successfully induced in a variety of animal species. Diabetes follows the alloxan induced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet beta cell, producing minimal effects on other structures. Alloxaninduced diabetes, a common model for evaluating. Furthermore, alloxan inhibits glucokinase, a shcontaining protein essential for insulin secretion induced by glucose. In the next step, rabbits were screened for diabetes. The purpose of this study is to evaluate the effect of ethanolic extract of cherry fruit on alloxan induced diabetic rats. Diabetes was induced in rats by intravenous injection of alloxan monohydrate dissolved in normal saline into the dorsal tail vein at a dose of 40mgkg body weight 6. Antidiabetic activity of vinca rosea extracts in alloxan. Effects of alloxan induced diabetes on ischemiareperfusion injury in rabbit hearts w. We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic activity of test compounds. Alloxan not only has toxic effects on islets of langerhans, but also affect other body organs.
G1 normal rats fed on normal diet, g2 diabetic rats fed on normal diet, and g3 diabetic rats were fed with raw camel milk. Research article alloxan induced diabetes mellitus in wistar. Alloxaninduced diabetes triggers the development of. Passiflora edulis, antidiabetic, hyperglycemia, alloxan mono hydrate introduction nsulin is the principal hormone secreted by. In alloxaninduced diabetic rats the ethanol extract 250 mgkg, p. The present study was carried out to evaluate the antidiabetic activity of vinca rosea methanolic whole plant extracts in alloxan induced diabetic rats for 14 days. Evaluation of alloxan on induction of diabetes in albino rats. The present study was designed to determine the neuroprotective effect of abelmoschus esculentus l. Alloxan diabetes definition of alloxan diabetes by medical. Diabetes was induced in wistar albino rats by intraperitoneal injection of alloxan 120 mgkg bw once. Avocado seeds may be of beneficial effects to diabetic patients. Introduction diabetes is a condition that arises when the. Animals received either saline c or alloxan 40 mgkg treatment on the day of proestrus pa preceding psp or on day 1 d1a of psp day 0 ovulation. Alloxan is most prominent chemical compound used in diabetogenic research.